Description
DL-Dithiothreitol(DTT, CAS: 3483-12-3, Synonyms: (DL-1,4-Dithiothreitol,1Msolutioninwater,formolecularbiology;Dithiothreitol,98%;DL-1,4-Dithiothreitol,DTT;DTTDL-1,4-Dithiothreitol;DL-Dithiothreitol,99%[forMolecularbiology];DL-1,4-Dithiothreitol,DNAse,RNAseandProteasefree,forMolecularb;DL-1,4-Dithiothreitol,DNAse,RNAseandProteasefree,forMolecularbiology,99+%1GR;DL-1,4-Dithiothreitol,forbiocheMistry,99%1GR) Dithiothreitol, commonly known as DTT (dithiothreitol), is a widely used reducing agent. It exhibits strong reducing power, largely due to the conformational stability of its oxidized six‑membered ring containing a disulfide bond.Its reducing ability is affected by pH and only functions effectively at pH values above 7. This is because only the deprotonated thiolate anion (–S⁻) is reactive, whereas the thiol group (–SH) is not; the pKa of sulfhydryl groups is typically around 8.3. requirements.
◆Chemical Properties
|
Melting point |
41-44 °C(lit.) |
|
Boiling point |
125 °C(lit.) |
|
density |
1.04 g/mL at 20 °C(lit.) |
|
vapor density |
5.3 (vs air) |
|
vapor pressure |
20 hPa (20 °C) |
|
refractive index |
0.019-0.29Pa at 20-50℃(lit.) |
|
Fp |
>230 °F |
|
solubility |
H₂O: 50 mg/mL, clear and colorless |
|
pka |
pK1:8.9 (25°C) |
| Biological origin | synthetic |
|
Odor |
Pungent odor |
|
PH Range |
4.0-6.0 (20-25℃, 0.1m in H2O) |
| λmax | λ: 260 nm Amax: 0.400λ: 280 nm Amax: 0.100 |
|
Sensitive |
Stable but heat-sensitive. Incompatible with strong oxidizing agents. Store at freezing temperatures between -20 and -10 °C. |
◆Applications
|
Sr. No. |
Description |
Application |
|
1 |
Reducing agent & biochemical reagent | Protein disulfide reduction & protein protection |
|
2 |
Biologically compatible reducing agent | Enzyme activity protection & biological sample stabilization |
|
3 |
Protective agent for sulfhydryl groups | Peptide synthesis & antibody / protein modification |
|
4 |
Reductive reagent for analytical chemistry | SDS‑PAGE sample preparation & chromatographic analysis |
|
5 |
Intermediate for pharmaceutical synthesis | API synthesis & impurity control in drug production |
◆Product Specifications
Dithiothreitol (DTT, CAS NO.3483-12-3) should be handled according to standard biochemical reagent and pharmaceutical intermediate safety procedures. Appropriate industrial hygiene and protective measures must be followed during handling, storage and operation.
- Use appropriate personal protective equipment (PPE)
- Avoid inhalation of vapor or mist and contact with skin, eyes and clothing
- Handle in controlled, well-ventilated environments
- Keep away from heat, sparks, open flames and hot surfaces
- Store in sealed containers, protect from moisture and oxidizing agents
- Follow chemical handling, storage and containment protocols
- Refer to Safety Data Sheet (SDS) for detailed safety, handling and storage information
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