It is white or almost white crystalline or powder; it is soluble in water, soluble in acid and alkali, slightly soluble in ethanol, and insoluble in organic solvents. It is a ribonucleoside (containing the pyrimidine base cytosine) that composes nucleic acid and constitutes a unit of the genetic code. Cytidine is paired with the corresponding guanosine in deoxyribonucleic acid and cytoribonucleic acid in a non-covalent form.
A preparation method of cytosine nucleoside includes the following steps:
①Silylation: N-(tert-butyldimethylsilyl)-2-(tert-butyldimethylsiloxy)-4-(tert-butyldimethylsilyl) is obtained by silanization reaction of cytosine and tert-butyldimethylsilyl chloride Pyrimidine amine, dissolve N-(tert-butyldimethylsilyl)-2-(tert-butyldimethylsiloxy)-4-pyrimidinamine in chloroform to obtain N-(tert-butyldimethylsilyl) Silyl)-2-(tert-butyldimethylsiloxy)-4-pyrimidinamine in chloroform;
②Glycoside synthesis: Dissolve tetraacetylribose in chloroform and add titanium tetrachloride solution. Add N-(tert-butyldimethylsilyl)-2-( at an ambient temperature of 18℃～30℃ (Tert-Butyldimethylsiloxy)-4-pyrimidinamine in chloroform solution, cytosine acetylribose is obtained by glycoside reaction;
③Ammonialysis: Add a methanol solution of 10% ammonia to the cytosine acetylribose at an ambient temperature of 18℃～30℃, and obtain the cytosine nucleus through the aminolysis reaction. Crude glycosides;
④ Refining: add the crude cytosine nucleoside to ethanol, heat to reflux under stirring, add water, cool to 0℃±2℃ and crystallize for at least 5h, separate the solid, and dry it to obtain the cytosine nucleoside.
